The Folio was studied extensively and it was determined that there was starch glue and vinyl glue areas located in the area of the staining, in most concentrated areas being right near the edge of the Folio. In addition, the presence of round inorganic nanoparticles about 100 to 200 nm in diameter, made up of mercury and sulfur was detected, which was accumulating within the cavities formed between the cellulose fibers of the framing paperwork